Biomedical products from Biomiga

PNGase F

Peptide NGlycosidase F, also known as PNGase F, is an amidase specifically remove asparagine-linked (N-linked) polysaccharides from glycoproteins.

Application: 

1. Proteomic analysis: MALDITOF mass spectrometry; identification of N-linked glycosylation sites.

2. Facilitate crystallization of glycoproteins.

Unit Definition:

One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One unit is equal to 1 IUB milliunit.

Adenovirus Purification Kit

Fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant.

Adeno-Associated Virus Purificat

Efficient purification of recombinant AAVs from rAAV vector transfected cell lines.

Lentivirus Purification Kit

Fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant.

Retrovirus Purification Kit

Fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant.

HCV Purification Kit

Fast and efficient purification of HCV from HCV-infected cell culture, plasma or whole blood.

Virus Concentration Kit

The kit provides an easy and cost-effective method for concentrating lentivirus particles without special equipment

Plasmid Miniprep

Isolate 50-80 ug of plasmid DNA in less than 30 minutes.

Plasmid Midiprep

Isolate up to 200 ug of plasmid DNA in less than 30 minutes.

Plasmid Maxiprep

Isolate 500-1000 ug of plasmid DNA within 60 minutes.

Plasmid Megaprep

Isolate 3-10mg of plasmid DNA within 60 minutes.

96-well Plasmid Miniprep

Rapidly purify plasmid DNA in a 96-well format using lysate clearance plates.

Endofree Plasmid Isolation

Isolate endotoxin-free plasmid DNA for high efficiency transfection

EndoFree ezFlow Plasmid Isolation

Isolate endotoxin-free plasmid DNA for high efficiency transfection in less than 60 minutes.

endA+ Strains

Isolate plasmid DNA from endonuclease(s)-contained bacterium.

High-Capacity Vectors

Isolate plasmid DNA from BAC, PAC, Cosmid and P1

Magnetic Beads Plasmid Isolation

A novel method for rapidly purify plasmid DNA in less than 30 minutes.

Uracil template preparation kit

This kit is designed to generate uracil containing DNA strand for site-directed mutagenesis

Yeast Plasmid Isolation

For the Rapid Isolation of Yeast Plasmid DNA.

Low Copy Number Plasmid Purification Kit

A novel method for purify plasmid DNA from low copy number plasmid

Vacuum Manifold

A novel method for rapidly purify plasmid DNA in less than 30 minutes.

Circulating DNA Isolation

Circulating DNA, RNA

FFPE DNA Purification

FFPE, formalin fixed parafilm imbedded

Mice Genotyping Kit

Mice Genotyping Kit - Mouse tail DNA ready in 30 minute!

Tissue gDNA Kit

Isolate genomic DNA from cultured cells and animal tissue.

Blood gDNA Kit

Isolate genomic DNA from blood sample, and can also used for buffy coat, serum, plasma, saliva, buccal swab and other body fluids.

Bacterial gDNA Kit

Isolation of genomic DNA, plasmids, BACs from bacterial samples.

Soil, Insect, Mullusc, Yeast and Mycoplasma

Isolate genomic DNA from Soil, Insect, Mullusc, Yeast, and Mycoplsma respectively.

Plant gDNA Kit

Isolation of total cellular DNA from plant cells and tissues.

Blood Viral DNA/RNA Kit

Isolation of DNA/RNA from blood virus.

Forensic gDNA Purification Kit

Isolation of genomic DNA from forensic samples, such as dry blood, buccal swabs, and sperm.

M13 Purifcation

Rapid and reliable isolation of high-quality M13 phage DNA from appropriate host strain.

Circulating DNA Isolation

Compared to other purification method in the market, this kit is simplified with only one lysis buffer and one wash buffer. The purified RNA is ready for Northern analysis, RT-PCR, MicroArray and real-time PCR. It is especially suitable for quantitative gene expression study.

Advantages

Fast and reliable: the whole procedure can be completed in 20 minutes

Stable RNA in DEPC water

No toxic organic chemicals

No genomic DNA contaminations

ChIP DNA Clean Up Kit

The Chromatin Immunoprecipitation (ChIP) DNA Clean Up Kit provides a simple method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol.

Isolate Nucleic Acid From Gels

Fast and reliable purify fragment DNA from Gel and Poly-Gel.

From Enzymatic Reaction

Purify target DNA /RNA probe from Enzymatic Reaction high efficiently.

Micro-volume Samples

Further purifying the yield DNA in a simple and rapid way

From Sequencing

Effective and reliable removal of unincorporated terminators from sequencing reactions.

2x SBGR qPCR Mix, Antibody Inactivated Hot Start

2xqPCR mix

2x SBGR qPCR Mix, Chemically Modified Hot Start

Chemically Modified Hot Start

DNA marker

Including a variety of DNA Ladders with sizes ranging from50 bp to 10 kb for use in agarose gel electrophoresis

PCR

The series contain all kinds of DNA polymerases, and dNTPs, 2x Taq PCR Mix

RT-PCR

Biomiga provides a series of M-MuLV Reverse transcription enzymes, can used for RT-PCR amplification,

Competent Cells

Including DH5a Competent Cells and DH5a Competent Cells in 96-Well Format

TumorGreen is a nanoparticle fluorescent in vivo blood vessel leakage imaging agent. TumorGreen enables imaging of circulation,blood vessels, vasculature, vascular leak, including that associated with various stage s of oncologic and opthalmologic lesions.

TumorRed is a nanoparticle fluorescent in vivo blood vessel leakage imaging agent. TumorRed enables imaging of circulation,blood vessels, vasculature, vascular leak, including that associated with various stage s of oncologic and opthalmologic lesions.

Cell line lysates

Including a variety of ready-to-use cell line lysates for proteomics research.

ELISA Kit

ElISA Kit and Reagents

Protein Marker

Inculding protein Marker and western-blot marker.

Protein Quantitation Kit

Protein Quantitation Kit

western blotting

Including ECL Western Blotting Substrate for Protein research.

Protein Purification

Including a series recombinated human and mouse proteins usually used in research.

Recombinant Proteins

Including a series recombinated human and mouse proteins usually used in research.

Antibodies

The set contains mouse anti-human and goat anti-mouse antibodies, which used widely in research.

GeneTranTM is a Polylipid-based reagent designed and formulated with proprietary technology for transfecting DNA or siRNA into a variety of eukaryotic cell lines, including HEK293, CHO, COS, NIH3T3, MCF-7, PC12, RKO, C2C12 myoblasts, mouse embryonic stem cells (ES) and fibroblasts (MEF), mesenchymal stem cells, NCCIT, S2 (Schneider) Drosophila cells as well as primary cultures of human carcinoma, rat adrenal chromaffin cells, mouse and rat cortical neurons, hepatocytes, and etc.  

GeneTranTM also offers extremely high transfection efficiencies and minimal cytotoxicity for suspension cultures including HEK 293, mouse lymphocytes, and insect cells (sf9, sf21, High-Five). The reagent works very well in cultures with serum concentration range from 0 to 20% FBS. 

Description

Dye-Mixed Low-to-High DNA Mass Ladder is suitable for use as a wide range of molecular weight standard for agarose gel electrophoresis (200 bp–10,000 bp). The DNA Ladder is composed of mixture of 14 DNA fragments of 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 800, 600, 400, and 200 bp. Electrophoresis of 5 µl of the Dye-Mixed Low-to-High DNA Mass Ladder results in bands containing 100, 80, 60, 50, 40, 30, 25, 20, 15, 100, 80, 60, 40, 20 ng of DNA, respectively. The concentration of DNA is ~0.144 µg /µl.

Dye-Mixed 100 bp DNA Ladder is suitable for use as a molecular weight standard (100 bp-1,500 bp) for agarose gel electrophoresis. The DNA Ladder consists of 11 DNA fragments between 100 and 1500 bp in multiples of 100 bp. The 500 bp fragment has increased intensity as a reference band. 

Five Minutes Competent Cell Preparation Kit

This kit enables researchers to prepare competent E. coli cells in a single step and to transform the cells without heat-shock. The competent cells obtained by this method can be kept at -70°C for long term storage. Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. Typical transformation efficiencies are 1E6~1E8 pfu/μg plasmid DNA. 

Advantages

Entire procedure takes about 5 minutes

Suitable for long term storage at -80°C

Reproducible

Cost effective: less than 10 cents /transformation 

Buffers

Including Buffers used in plasmid DNA, DNA and RNA isolation kits

Chemicals

Sodium Chloride, Tris, Sodium Phosphate 

FACS Application

Including sterile or non-sterile tubes, Cell Culture Grade Water and FACS Buffer

General Lab Supplies

The set including Bottles, Gloves, Parafilms, Pipette, etc. widely used in bio lab

Molecular Biology Application

Including some widely-used molecular biology applications

Protein Chemisitry Application

Including Tween 20, SDS-PAGE Protein standard, Dialysis Bag, etc. which were used widely in biolab